Experimental Investigation of Ultrasonic Trapping of Bioparticles Using Parallel Plane Cavity

This paper explores the use of acoustics for manipulation of bioparticles. A series of experimental studies were conducted using acoustic standing waves in resonant cavities as means of trapping bioparticles and enhancing bioparticles separation. Parallel Plane Cavity was explored. The effect of cell size and host media concentration on trapping efficiency, trapping time and velocity were investigated. Monitoring of the cells behavior during ultrasonic manipulation by using three simple techniques (light microscopy, dielectric measurement and optical absorption technique) was studied. Study indicates that increasing ultrasound intensity increases the trapping efficiency of cells and decreases the time consumed to trap cells. Also force increases with increasing cell diameter. Increasing of cell media concentration tends to increase the trapping time and decrease the cell velocity. Osmatic fragility studies of the trapped cells indicate that there is no effect on cell membrane integrity of the biological cell exposed to ultrasonic standing wave.